BIOL 2020 Study Guide - Final Guide: Pyruvate Dehydrogenase, Acetyl-Coa, Lipoamide

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11 Feb 2013
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Converting pyruvate into acetyl coa, and producing 2e and co2, decarboxylation, oxidation, and transfer of coa group, coupled to conserve energy. Large, 3 enzymes, each has their own active site. Pyruvate combines with carbanion to tield hydroxyethyl tpp, catalyzed by e1. Hydroxyethyl group oxidized to form acetyl group transferred to lipamide(disulfide group) reduced to disulfhydryl by e1. Acetyl group from acetyllipoamide transferred to coa, e2 is used, thio bond is conserved. 8 e2, 24 e1 (a2ba), 12 e3 (ab) Amino (flexible lipoamide cofactor), lipoamide, transacetylase coordinated catalysis: converting pyruvate into acetyl. Neurological b2 deficiency: nadh is final product. Nadh, acetyl coa, atp vs. nad, coa, adp. Low levels of thiamine (b1) , can be inherited, loss of feeling and muscles. Madhatter symptoms, reduce sulfhydryl on e3, treatment : sulfhydryl reagents. They have less glucose consumption in the brain, because there b1 is a coenzyme of.

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