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BIOL3110 Exam study guide.doc

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BIOL 3110
Peter Cheung

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BIOL3110 Exam study guide: st  Check out your 1 two tests  What is tautomerization and how does it affect H bonding between bases?  How does the structure and atom numbering differ between purines and pyrimidines?  The synthesis of nucleotides are regulated by several feedback mechanisms. Which enzymes are regulated by feedback? How are deoxyNTP’s synthesized?  How are chemotherapeutic drugs able to kill cancer cells?  What is the difference between a Northern and a Southern hyb? Can a Southern be used to examine the regulation of expression of a gene? Can a Northern? How could you label a probe radioactively or fluorescently?  DNA polymerization occurs by a specific molecular mechanism. What are the steps involved? Can uracil be found in DNA? How could this happen and how could it be fixed? What id fidelity? Processivity?  How is DNA sequencing performed? How is a sequence read and how could you identify a potential ORF? How does a pseudogene differ from an active gene? Does base sequence affect protein binding? What is the +1 base in transcription?  Methylation of DNA occurs within bacterial cells – some mutants lack this ability. With respect to repair, what might occur if a cell lacked the ability to methylate adenine? How might eukaryotes differentiate the template and nascent strand for repair purposes? Why is this important with respect to genetic fitness?  How is new recombinant DNA put into host cells? How are antibiotics and nutritional requirements used to select transformed cells?  What are the steps involved in making libraries? Genomic and cDNA placed in to phage or plasmid vectors (dependant on size) by ligation and then transformed into cells. Selection is determined by lac Z and amp resistance. What are the pro’s and con’s of each kind of kind of library? What factors affect hybridization of nucleic acids? Can mutations be identified by hybridization?  What affects annealing - salt, temp. High salt, low temp = high or low Stri
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