BIOL3110 Exam study guide: Partial – might be revised later. MIGHT!! Revision 1
Check out your 1 two tests
What is tautomerization and how does it affect H bonding between bases?
How does the structure and atom numbering differ between purines and pyrimidines?
The synthesis of nucleotides are regulated by several feedback mechanisms. Which
enzymes are regulated by feedback? How are deoxyNTP’s synthesized?
How is Salvage different from de novo? In which pathway would you find ribonucleotide
reductase? HGPRT? DHFR? Do salvage enzymes or related diseases exhibit any tissue
How are chemotherapeutic drugs able to kill cancer cells? What works against them?
How does imprinting affect disease?
Bromo and chromodomains. Purpose? How have they been implicated in cancers
(animation and the medicine article)
DNA polymerization occurs by a specific molecular mechanism. What are the steps
involved? Can uracil be found in DNA? How could this happen and how could it be fixed?
Coo forms a bridge b/w the nascent strnd and the incoming nucleotides. Also the
magnesium stuff, and how it stabilies the phosphates! If cytosine is deaminated it will form
uracil. When thymidylate synthetase is not working, dtmp nott made and lead to
accumulation of dump. Uracill dna glycosylase has edonuclease activity which will chop out
the u. exonuclease excision of singke strad patch, re-replicstin of patch by repair
polymeasrse by using other stand as a tempate.
How is DNA sequencing performed? How is a sequence read and how could you identify
a potential ORF?
DNA and histones can be altered by various enzymes - (Like?) - what is the result of
these various alterations?
Hat, hdac, kinase. Hat acelytaion recognized boromo, then once recongnized attract
proteins swi/snf remoledlling that exposes tht tata box. Hdac, on slides. Serines
phosphoryaltion causes condensation.
How is new recombinant DNA put into host cells? How are antibiotics and nutritional
requirements used to select transformed cells?
Cut with the same re on plasmid and dna of interst, ligate them and transform them into
ecoli cells. There are selectable markers pick an ecoli cell which is a mutant, so it doesnt
grow the antibiotc or medium without histidine, put thpse cells on a plate with the antibiotic
or in the absene of nutrient and if they grow that means they have the plasmid, since cells
that have the plasmind would survive in amp or media wi