KINE 4450 Study Guide - Midterm Guide: Skeletal Muscle, Elisa, Fibroblast
1 Mar 2017
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Hypoxia affects all aspects of process vegf: +hur: stabilizes vegf mrna to stop degradation. Reason to study angiogenesis: angiostatic factors: physiological processes (exercise training, altitude, menstrual cycle, angiogenic factors: pathological processes (ischemia - want more stimulation, tumour development - too excessive, want more inhibition) Pcr: measure expressed mrna, amplification of specific dna sequence, several amplification cycles, reactions catalyzed by taq dna polymerase - remains active at. Makes copies of dna vegf to increase amount of desired gene, then visualise it; only copying small area of dna sequence that has the gene. Isolation of mrna (muscle sample muscle solution rna) and reverse transcription into cdna (different from original dna because don"t have introns, they were removed) Vegf is 46kda normally, should be 21-23kda on gel. Smaller proteins move farther down and faster, bigger ones stop because too big: electrotransfert: proteins on gel are transferred onto nitrocellulose membrane or pvdf, makes photocopy of proteins, current passes through horizontally, transfers protein to sheet.
