BIOL3040 Study Guide - Comprehensive Midterm Guide: Fluorescence Microscope, Lipid Bilayer, Prnp

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7 Feb 2017
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BIOL 3040
MIDTERM EXAM
STUDY GUIDE
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JANUARY 18 LECTURE 1
Class Expectations
- OH: M 1-2, T 12-1 EXTRA CREDIT POINT FOR GOING TO OFFICE HOURS
- All powerpoints are in modules and lecture capture for all the classes
- After class surveys: 2-4 uestios cofusig/liked/eta stuff optioal fo ee  sues, ou’ll
get an extra point added to grade)
- Textbook: supplemental learning
o Everything on tests will be said in lectures
o Free online version
- All of last ea’s quizzes/finals/midterms on bottom of modules
o Keys will not be posted until 2 days before midterm/final
- 2 optional extra credit projects
o Create a movie or a song that illustrates a cell biological process (self or group)
o Write a practice midterm question
- blue text is vocabulary
- purple text is for supplemental benefit
LECTURE: How we study cells (EUKARYOTES ONLY)
- Microscopy
o looking at cells in organism and petri dishes through a microscope
o Hooke coined cell theory when studying corks
- Light Microscopy
o Using light to illuminate a sample
o Bright field: have light, put light on a sample, and look
Required cells to be fixed (not moving and dead)
Stained after killing the cell
o Phase contrast, DIC are used to identify different parts of the cell
o Limit of resolution (Diffraction Limit)
Thee is a distace at hich ou ca’t tell  spots (or proteins) apart
Distance at which you can resolve 2 spots (point at which you can no longer tell 2
proteins apart)
200nm
- Fluorescence microscopy
o Tagging a protein in the cell to identify the location of the protein in the cell
o 2 ways
Live imaging: Express a protein that is tagged with a fluorescent molecule such as
GFP (fluoresces) (Green fluorescent protein)
Immunocytochemistry: fi the cell, the add antibodies that bind to proteins,
then add fluorescent molecules to the antibodies
- Electron microscopy
o Uses electrons to ************
o Cells are dead to image them
o 2 kinds
Transmission EM: ********
Looks inside the cells
Electrons are shown through a slice of a cell?
Scanning EM: Coat outside of cell in a metal, hit electrons onto the surface to create
a 3D resolution
Looks outside of the cell
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JANUARY 18 LECTURE 1
- Model Systems
o used to study cell function
o best to pick simplest model system to answer your question regarding cell biology
bree’s east has the siplest sste fo diisio
Worms have the simplest system to study neurons
Zebrafish have the simplest system to study **********
- Cell culture (tissue culture)
o Take out a neuron, it will retain the properties of the neuron
o needs to have sustainable environment to grow and not die
o ********
- Question:
o What are the pros and cons of using live imaging of protein tagged with GFP versus
Immunocytochemistry to visualize a protein in a cell?
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