BIOL 311 Study Guide - Midterm Guide: Atomic-Force Microscopy, Scanning Tunneling Microscope, Ion Exchange
11 Mar 2018
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Document Summary
Atomic force microscope (afm): scans single cells and creates an image. Scanning tunneling microscope (stm): claim to fame is it is the first one to visualize dna. Two photon microscopy: when compared to confocal, it allows for deeper tissue penetration and it generates less heat. Cell separation: used to separate a cell of interest from the others. Protein purification (enrichment): lyse the cell and release organelles. Can be done through: ion exchange chromotogrophy, gel filtration, affinity chromatography, sds gel electrophoresis, native gel electrophoresis, western block, protein-a immunization, protein chips, seldi-tof, maldi-tof. Fluorescense activated cell sorting (facs): machine that requires that you label cell x w/ a fluorescent probe. Guava: small sample quantitative machine that doesn"t separate cells but does count them. Microfluidic devices: dynabeads: inside of the cell is iron, you bind the cell to an antibody, Then use a magnet to separate the cells. Veridex cell search system: circulates out ctc"s: fda approved for clinical application:
