BIOL 311 Study Guide - Midterm Guide: Autoradiograph, Electron Tomography, Transfection

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Fret: can be used to determine if two proteins interact in vivo. Fluorescence resonance energy transfer: autoradiography: the use of isotopes to track a variety of cell processes. Tells if cells are actively dividing: fish: fluorescence in sito hybridization: allows you to search for the presence of genes or expression of select mrna"s. Can be used to perform chromosome painting which helps in differentiating chromosomes of similar size and shape. Intracellular injection: single cell microinjection: pipette is the size of a mitochondria. Disadvantage is it only does one cell at a time. The four intracellular injection techniques are single cell injection, electroporation, liposome based injection and gold particles techniques. Single cell injection can be used for somatic cell nuclear transfer where you inject a cell with a pipette the size of a mitochondria. It"s disadvantage is that it only does one cell at a time. Dna into the cells by using an electric field to creates holes in the membrane.