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CAS BI 203 Study Guide - Quiz Guide: Taxus Baccata, High-Performance Liquid Chromatography, Nuclear Magnetic Resonance

Course Code
CAS BI 203
Martin Steffen
Study Guide

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Cesar Diaz
Bio Discussion 9- Taxol Cytoskeleton Cell Cycle Cancer
Part I
1. What type of plan could be carried out by adding the seeds of Taxus baccata to a pecan pie?
The intruder could be trying to poison someone. By adding the Taxus Baccata seeds to the pie, it
would have caused the pie to become bitter with taste, numbing the persons’ taste buds, infecting
their blood stream and thus killing them.
2. What are taxines?
Taxines are the active, position constituents in yew plants and have been implicated in animal and
human poisonings. Several taxine alkaloids have been isolated and characterized through the use of
high performance liquid chromatography, mass spectroscopy, and nuclear magnetic resonance
Part II
3. Most of the goeror’s lunch party just ate pecan pie fi lled with taxines, a potent mixture of
poisons, one of which is actually a powerful mitotic inhibitor. What is a mitotic inhibitor?
A meiotic inhibitor is a drug that inhibits mitosis, or cell division. These inhibit mitosis by disrupting
the microtubules, which are structures that pull the cell apart when it divides.
4. Which specific cytoskeletal element is most susceptible to mitotic inhibitors?
The cytoskeletal element most susceptible to miotic inhibitors are microtubules.
5. Describe the function of mitosis in animal cells. Why is mitosis important?
Mitosis is a process of cell division. The purpose of it is tissue growth, regeneration, or asexual
reproduction. It is important because it is essential for the growth and repair of the body.
Cell Cycle
Interphase Long period of cell cycle during which DNA replicates, the centrioles divide, and
proteins are actively produced.
Prophase First miotic stage in which the nucleolus fades, nuclear envelope breaks down, and
chromatin condenses into chromosomes
Prometaphase- Chromosomes continue to condense, kinetochores appear at centromeres,
miotic spindle microtubules attach to kinetochores, and centromeres move toward opposite
Metaphase- Miotic spindle is fully developed, centrosomes are @ opposite poles of cell,
chromosomes are lined up at the metaphase plate, each sister chromatid is attached to spindle
fiber from opposite poles.
Anaphase Cohesin proteins binding sister chromatids break down, sister chromatids
(chromosomes) are pulled toward opposite poles, non-kinetochore spindle fibers lengthen,
elongating the cell.
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