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Match each term with its definition. Each term or definition is used only once
right-spiraling common DNA form
referencing the opposite orientation of hydrogen-bonded DNA strands
viral particle whose natural host is a prokaryote
universal holding that the number of purines always equals the number of pyrimidines
nucleic acid base sequences able to form a double-stranded structure by virtue of matching base pairs
5ââ 3â or 3ââ 5â
nuclease able to cleave phosphodiester linkages in the DNA backbone
the structure formed by two DNA strands twisted around a common axis in a spiral
bacterial cycle of phage- infected cells resulting in cell destruction and viral release
main access site for proteins that recognize specific DNA sequences
a cytosine modification favoring Z DNA formation
nucleic acid monomer consisting of a sugar, a phosphate group, and a nitrogenous base
connects two nucleic acid sugar molecules via an ester bond
hydrolyzes peptide bonds linking amino acids
U or C in RNA
a pentose sugar
an enzyme that breaks down RNA
either of the two nucleotide chains of a double helix
a pyrimidine able to hydrogen bond with adenine
a DNA configuration favored by high ionic strength and GC repeats
Don appropriate personal protective equipment (PPE).
Aseptically transfer 1 mL from dilution tube 2 to dilution tube 3 and mix well. This is a 10-5 cumulative dilution.
Aseptically transfer 1.0 mL from dilution tube 4 to dilution tube 5 and mix well. This is a 10-7 cumulative dilution.
Aseptically transfer 0.1 mL from dilution tube 1 to dilution tube 2 and mix well. The cumulative dilution of the culture is 10-4.
Allow the inocula to soak into the plates before proceeding.
Aseptically transfer 0.1 mL from dilution tube 2 to plate A1. Using the spread plate technique, disperse the sample evenly over the entire surface of the agar.
Aseptically transfer 1 mL from dilution tube 3 to dilution tube 4 and mix well. This is a 10-6 cumulative dilution.
Obtain eight plates, organize them int four pairs and label them A1, A2, B1, B2, etc.
Keep accurate records in your lab notebook.
Clean up the lab benches, doff PPE, and wash hands.
Repeate the procedure with plate A2 and label both plates "10-5mL of original sample," or simply "10-5"
Notice that transferring 0.1 mL counts as a dilution!! The tube was a 10-4 dilution, but the plate is a 10-5 dilution!!
Invert the plates so they are "agar up" to prevent condensation from ruining the plates.
Repeat the aseptic transfer, spread plate technique, and label schematic for dilutions tubes 3, 4, and 5, and plates B, C, and D.
Incubate the plates at 35â for 24 to 48 hours.
Obtain five dilution tubes and label them 1â5, respectively. Make sure they remain covered until needed.
Aseptically add 9.9 mL sterile water to dilution tubes 1 & 2. Cover when finished. Aseptically add 9.0 mL sterile water to dilution tubes 3, 4, and 5. Cover when finished.
Mix the broth culture an aseptically transfer 0.1 mL to dilution tube 1. Mix dilution tube 1 well. This is a 10-2 dilution