PCB 2061- Midterm Exam Guide - Comprehensive Notes for the exam ( 32 pages long!)
Document Summary
Which statement is true in regard to eukaryotic transcription-linked nucleotide excision repair. The large subunit of rna polymerase is degraded; tfiih remains and recruits xp proteins to repair dna damage. In ber, polymerase delta and epsilon replaces long stretch of nucleotides, which is 1500-9000 bases. *correct answer is replace 2-10 bases aka long patch repair. The uvr-/reca- double mutants can tolerate up to 50 thymine dimers. Msh2/msh3 complex binds dna loops resulting from replication slippage. Msh2/msh6 complex binds single base mismatches, while other proteins do the repairing. Msh repair system in yeast is homologous to the e. coli muts/l system. Roles of muts and mutl are completely different between bacterial and msh eukaryotic proteins. Dam methylation system marks the gatc sequence in the original strand and unmethylated daughter strand is repaired. Reca cuts lexa thus inhibiting the inhibitors activity. A replication fork may stall when it encounters a mismatch.
