MCB 52 REVIEW NOTES
[LECTURE 31] EXAMINING NOISE IN LOW COPY PLASMIDS
We want to use a weak promoter, because difficult to detect noise when there is so
much signal. You want each cell to only have one copy of each promoter. They also
didn’t want the proteins to be too close to each other (as they would be if they were
on the same plasmid).
Experiment 1: Took cells from WT or FH patients. In wild-type patients, cholesterol
led to a decrease in HMG Co-A reductase activity, but in FH patients, you didn’t get
that decrease in activity.
NONSENSE, NONSTOP, NO-GO
Nonsense: a premature stop codon has been introduced
Nonstop: stop codon has been mutated, once ribosome starts translating the polyA
tail, complexes are degraded.
No-go: Ribosome becomes stalled, possibly because of