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BIOL 400 Study Guide - Midterm Guide: Dna Polymerase Iii Holoenzyme, Lac Repressor, Dna Polymerase IExam

Course Code
BIOL 400
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EXAM CODE 444444
1. Proteins that are responsible for activation or repression of protein encoding genes are called
___________ proteins.
a. Inducer
b. Regulatory
c. Sigma
d. Tus
e. Rho
2. Which of the following is NOT required for lagging strand synthesis?
a. DNA Primase
b. DNA Polymerase III
c. DNA Polymerase I
d. Ligase
e. RNA polymerase
3. Which of the following statements regarding the lactose repressor is/are true?
a. The lactose repressor is present in the cell only when lactose is present.
b. The lactose repressor protein is always being expressed.
c. The lactose repressor binds to the -35/-10 region of the lac promoter.
d. The lactose repressor binds to the structural genes of the lac operon.
e. Two of the above statements are true.
4. A mutation in the DNA that results in a different amino acid in the encoded protein is termed a ______
a. Silent
b. Missense
c. Nonsense
d. Readthrough
e. Stop
5. Which enzyme is responsible for removing the RNA primer on both the leading and lagging strand?
a. Helicase
b. RNase H
c. Ligase
d. DNA polymerase III
e. Gyrase
6. Which enzyme forms a phosphodiester bond between Okazaki fragments during fragment resolution on
the lagging strand?
a. Rnase H
b. Helicase
c. Ligase
d. Topoisomerase
e. DNA polymerase III
7. Which of the following bases are found in DNA molecules?
a. Cysteine, guanine, uracil, thymine
b. Guanine, adenine, thymine, cytosine
c. Glycine, tyrosine, arginine, cysteine
d. Adenine, thymine, cytosine, uracil
e. Adenine, thymine, cysteine, glycine
8. _______ synthesizes a short stretch of RNA complementary to the parent strand to initiate synthesis of
DNA by DNA polymerase III.
a. DNA polymerase I
b. RNA polymerase
c. DNA primase
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d. Rnase H
e. Ligase
9. This enzyme is responsible for unwinding the double stranded DNA into single stranded templates for
a. DNA polymerase I
b. Ligase
c. Helicase
d. Gyrase
e. Topoisomerase
10. A polycistronic mRNA with six protein coding genes has:
a. 1 start codon, 1 stop codon, and 1 Shine-Dalgarno sequence.
b. 3 start codons, 3 stop codons, and 3 Shine-Dalgarno sequences.
c. 1 start codon, 6 stop codons, and 1 Shine-Dalgarno sequence.
d. 6 start codons, 1 stop codon, and 6 Shine-Dalgarno sequences.
e. 6 start codons, 6 stop codons, and 6 Shine-Dalgarno sequences.
11. Initiation of DNA replication begins when _______ bind(s) to the ______.
a. Sigma; promoter
b. RNA polymerase; sigma
c. Ribosomes; Shine-Dalgarno sequence
d. DnaA; oriC
e. Tus; Ter
12. Amino acids are covalently linked to a tRNA molecule by _________. These tRNA molecules are said to be
a. tRNA polymerase, loaded
b. aminoacyl-tRNA synthetases, charged
c. tRNAses, activated
d. aminopeptidases, linked
e. peptide bond, reduced
13. The Shine-Dalgarno sequence ________.
a. Is a site on the DNA for the ribosome to bind.
b. Is located on the mRNA upstream of the start codon.
c. Is located on the mRNA upstream of the promoter.
d. Is located on the mRNA downstream of the terminator.
e. Is located upstream of the transcription start site on the mRNA.
14. The term “operon” describes____________.
a. The promoter region of a gene.
b. Multiple genes transcribed from a single promoter.
c. The placement of an Okazaki fragment within the spacer regions on an mRNA.
d. A single gene transcribed by multiple polymerases.
e. The site on a gene where regulatory proteins bind.
15. The term “regulation of gene expression” refers to the process(es) that control___.
a. Transcription and replication
b. Transcription and translation
c. Replication and translation
d. Replication, transcription, and translation
e. Transcription only
16. Which of the following would not be found on a gene that encodes for a tRNA molecules?
a. A promoter
b. A Shine-Dalgarno sequence
c. The start site for transcription
d. A -35/-10 consensus sequence
e. A rut site
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