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BIOL 1201 Study Guide - Quiz Guide: Dna Ligase, Prokaryote, Stop Codon


Department
Biological Sciences
Course Code
BIOL 1201
Professor
E.Wischusen
Study Guide
Quiz

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Review Exam 3
ATP Synthetase
If you decrease pH the you make more ATP.
This will not effect NADPH
If you change the pH outside the thylakoids then it effects the amount of ATP produced.
More H in the thylakoids the more free energy inside them.
If you increase the gradient then more ATP is made because the H have more free energy
Increase of H then they will have more energy when they exit the ATP synthetase
DNA Replication
DNA Polymerase attaches at the origins and builds the new piece towards 5 to 3 (right to left)
and antiparallel. Leading strands
Lagging strands build towards 5 to 3 (right to left) and antiparallel.
Replication happens opposite to the direction the bubble opens.
Helicase- Separates the backbones and unwinds the helix creating the bubble
Topoisomerase- relieves the stress, lets the molecule unwind
Primase- puts nucleotides on the backbone
SSB Proteins- holds the backbones apart, are at the forks
DNA Polymerase- binds to primer and starts replication
DNA Ligase- Joins the backbones together
Replication bubble grows, transcription bubble slides
Phases
Replication DNA-DNA nuclear in Eukaryotes
Transcription DNA-RNA….. akes oe gees oth of RNA nuclear in Eukaryotes
Translation RNA-Protein Cytoplasmic in EUK
Transcription
Matching complementary DNA to nucleotides
Promotor- tells the genes where to start and which backbone to start with
Pay attention to directionality!!!!!!!!!
Most common promotor has the TATA box
TATA seuee is o the akoe that is’t tasied
Has RNA Polymerase
Translation
Fist triplet that reads AUG starts the translation
tRNA MOLECULE THAT HAS THE APPROPRIATE NUCLEAOTIDES BIND SAND BRINGS METHYANINE
THEN THE RIBOSOMAML SUB UNIT SITS ON IT
Ribosome has 3 sites E, P, and A
Ribosome shifts 3 nucleotides 5-3
Stop codon removes ribosome and releases the sequence of amino acids
Gene Regulation
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