BIO 202 Study Guide - Midterm Guide: Chemotaxis, Integrin, Desmosome
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What are the units of measurement in histology: Resolving power: smallest distance between two things such that you can distinguish the difference between those two things. (2 separate points) Fixation: using gluteraldehyde or formaldehyde to cause separate proteins to bind with each other. (this is an aqueous solution) Dehydration: this occurs as a series over the course of a few hours. The tissue sample is soaked in ethanol baths ranging from 10% ethanol to 100% ethanol. Clearing: use of xylene to clear out ethanol because of ethanols properties that are similar to that of water. It is embedded with paraf n or plastic for higher resolution. This is done in a series as well/ the wax replaces the xylene. Xylene is a liquid at room temperature, whereas wax is a solid at room temp. Sectioning: the tissue is is shaved with a microtome to sections that are thousandths of an inch thich.
