GENE 540 Midterm: Cell Free Mitosis
Document Summary
This is cell cycle for a non-degradable cyclin b the cell arrests in late anaphase because cyclin. B is not degraded, hence it doesn"t leave mitosis. Conclusion: cyclin b destruction is required for exit from m-phase. In this extract, we are adding an inhibitor for e3-ubiquitinated ligase (apc) this causes the mitosis to stay at metaphase and not go to anaphase. Hence, apc is required to go from metaphase to anaphase. Complex (apc) we need this e3- ubiquitinated ligase to target some protein for destruction to allow cell to go from metaphase to anaphase. Cohesin has to be proteolyzed to go from metaphase to anaphase. The picture indicates that apc (the e3 ubiquitin ligase) is required for anaphase and again later to degrade cyclin b to m-phase. So apc targets securins for proteolysis securin is degraded so that separase can no longer be inactive this is the trigger to go from metaphase to anaphase.
