GENE 540 Study Guide - Midterm Guide: Origin Recognition Complex, G2 Phase, Dna Replication
Document Summary
There is assembly of the pre-rc and then we have cycline-cdk2 + cyclina-cdk2 causes replication to happen. S-phase are available: cdk2/cyclin e and cdk2/cyclin a. Cdk-cyclin activity prevents the assembly of the pre- replicaton complex. It says that the origin recognition complex (orc) proteins re-bind the origin, however, the other pre- replication complex proteins (cdc6, cdt1, mcms) do not bind this is due to the cdk/cyclin activity. As long as there is cdk/cyclin activity you cannot reassemble the pre-replication complex activity. The block to re-replication is mediated by s phase and m phase cdk/cyclin activities, which inhibit assembly of pre-replication complexes. There is one point (right after the cyclins degraded") where cyclins are degraded and there are no cyclins anywhere at this point of the cycle. Due to apc activity, all cyclins are gone at m/g1 (and not at any other time). But, the pre-replication complex can only form when all the cyclins are gone at the.
