GENE 540 Midterm: Cell Cycle Proteolysis & Spindle Checkpoints
Document Summary
The proteolysis at specific times is a major contributor to keep the cell cycle going to one direction. Proteolysis is pretty much irreversible: this helps the cell cycle to go in one direction. Scf with other targeting subunits are responsible for normal cyclin e cycling. D this would lead to the cell not passing through the restriction point. So if you made lots of p16 it would inhibit cdk4/cyclin. In this experiment they are expressing p16 when p16 is expressed, the cell gets arrested at. The mutant p16 cannot bind to the cdk, so you still get cells going from g1 to s phase. The graph on the right shows p16 being injected into fibroblast and then you see cells not going into. S-phase; however, if you inject p16 into a cell that has mutant rb-gene, then the cells will go into s-phase (hence, a bunch of p16 wouldn"t matter if you didn"t have rb)