Bio Exam 3: Cell Respiration

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Department
Biology
Course
BIOL 1101
Professor
Mary- Susan Potts- Santone
Semester
Fall

Description
Cell Respiration Flashcards/Study Guide Cell Respiration Equation ­ ­C6H12O6+6O2====>6H2O+6CO2  ­27­29 Net gain ATP and Heat ­DeltaG=­686 KCAL/Mol (exergonic) Cell Respiration Parts ­ Glycolysis, Krebs (CAC) Cycle, Oxidative Phosphorylation Glycolysis inputs and outputs ­ ­Glucose, 2NAD+, 2 ATP, 4 ADP+4 P ­2 Pyruvate, 2 NADH, 2 ADP, 4 ATP ­2 ATP NET GAIN Glycolysis Process ­ ­glucose splits into 2 G3P molecules using 2 ATP  ­2G3P converted to 2 Pyruvates ­2NAD+ reduced to 2 NADH ­4 ATP produced in substrate level phosphorylation Transition/Prepatory Reaction ­ ­Converts pyruvate to 2 acetylCoA ­2 Pyruvate+2CoA===> 2 acetyl CoA+ 2 CO2 ­2 NAD+ is reduced to 2 NADH ­CO2 is released and transported to cytoplasm Krebs Cycle ­ Happens in Mitochondria and runs 2x per glucose 1)acetyl CoA combines with oxaloacetate, creates 6­C citrate 2) NAD+ reduced to NADH +H+, CO2 is released 3) ATP produced (substrate level Phosphorylation) 4)FAD is reduced to FADH2 when succinate==>Fumarate (oxidized) Oxaloacetate=>Citrate(6)=>Ketoglutarate(5)=>Succinate(4)=> fumarate=>Oxaloacetate Inputs outputs of Krebs Cycle ­ 2 Acetyl CoA, 6 NAD, 2 FAD, 2ADP+2P 4CO2, 6NADH, 2FADH2, 2ATP substrate level phosphorylation ­ in pyruvate kinase Oxidative phosphorylation location ­ Cristae of mitochondria or plasma membrane in  prokaryotes Oxidative phosphorylation ­ ­Electrons from NADH+ and FADH2 travel down electron  carriers (cytochromes) to final electron acceptor 1/2O2 ­Oxygen picks up 2 electrons and 2 H+ ions to form H2O ­1/2O2+2H+ +2e­==>H2O ­Energy released in redox reactions pumped H+ into innermembrane space and creates  H+ gradient (Proton motive force) ­chemiosmosis: H+ ions diffuse back through inner membrane, ATP created Energy yield per glucose molecule ­ ­reactants:696 kCal ­energy yield (original): 36 ATP (263 kCal) efficiency = 38% ­Energy yield (correct): 27 ATP (197 kCal) efficiency=29% how much ATP formed each stage ­ ­Glycolysis: 4 ATP; Net gain 2 ATP ­Krebs: 2ATP ­Oxidative phosphorylation: 23­25 ATP types of poisons that stop cell respiration ­ ­Block ETS ­Respiratory poisons ­Uncouplers Poisons that Block ETS ­ ­Rotenone: Binds with electron carrier in first protein complex  (for insects.pests, fish) ­Cyanide, CO: binds with cytochrome c, blocks passage of electrons to O2 Respiratory Poisons ­ Inhibit ATP synthase ­Oligomycin, malachite gre
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