🏷️ LIMITED TIME OFFER: GET 20% OFF GRADE+ YEARLY SUBSCRIPTION →
Log in
HomeStudy Guides420,000US230,000

33:011:100 Midterm: Term Test 2 - Fall 2017

67 views27 pages
School
Rutgers University
Department
Administrative Studies
Course
33:011:100
Professor
Ken Genco

For unlimited access to Study Guides, a Grade+ subscription is required.

Document Summary

Employee behavior: the pattern of actions by the members of an organization that directly or indirectly influences the organization"s effectiveness. Counterproductive behaviors: behaviors that detract from organizational performance. Personality: the relatively stable set of psychological attributes that distinguish one person from another. Individual differences: personal attributes that vary from one person to another. Emotional intelligence: the extent to which people are self-aware, can manage their emotions, can motivate themselves, express empathy for others, and possess social skills. Attitudes: a person"s beliefs and feelings about specific ideas, situations, or people. The extent to which people have positive attitudes toward their job: organizational commitment. An individual"s identification with the organization and its mission. Psychological contract: the set of expectations held by an employee concerning what he or she will contribute to an organization and what the organization will provide the employee in return. Person-job fit: the extent to which a person"s contributions and the organization"s inducements match one another.

Get access

Grade+20% off
$8 USD/m$10 USD/m
Billed $96 USD annually
Grade+
Homework Help
Study Guides
Textbook Solutions
Class Notes
Textbook Notes
Booster Class
40 Verified Answers

Related Documents

33:011:100
Final Exam
Study Guide
33:011:100 Study Guide - Midterm Guide: United States Dollar, Comparative Advantage, Price Drop
cerisegiraffe538
33:011:100
Final Exam
Study Guide
33:011:100 Final: Complete and Comprehensive 85-Page Final Exam Study Guide!
cerisegiraffe538
33:011:100 Lecture Notes - Lecture 1: Time Management, Collective Bargaining, Decision-Making
raspberryhamster349

Related Questions

Need help with this study guide for my calculus 2 midterm. Please solve problems and show the solutions step by step for each problem. .

For Discussion (2-3 paragraphs) • What was the concentration of your pre-diluted NPs? • Determine the effect of NPs on brine shrimp lethality. • Determine the effect of NPs on cell viability. • What is (are) the control(s) in this experiment? What is its purpose? • Based on your data in this lab, what is a safe concentration for brine shrimp Threshold Level of Toxicity (TTL)? • Often indicator species are used to study the overall health of an ecosystem. If you were to study an ecosystem containing brine shrimp, would you use it as an indicator species? Why or Why not? Explain your reasoning. • What possible sources of error were present in this experiment?

Introduction

LD50 is a commonly used term to describe acute toxicity: LD stands for Lethal Dose and 50 is used because the dose could kill 50% of the animals exposed to the chemical. For LD50 tests, the animals are given specific amounts of the chemical, orally, by injection or dermally. The value is then reported along with the method of administration and the type of animal used in the test. LD50 is typically reported in milligrams or grams per kilogram of body weight of the animal. The lower the LD50 is, the higher the toxicity of the chemical would be. This information is always provided in Safety Data Sheet (SDS) of chemicals in the “toxicity data” category. A similar measure, the LC50 is usually used to describe the ecological hazards of chemicals. LC stands for lethal concentration that could kill 50% of the animals after 24-96 hours. This value is always provided in Safety Data Sheet (SDS) of chemicals in the “Ecological Data” category. In this lab, you will be measuring LC50 or LD50 of iron oxide nanoparticles using brine shrimp. Iron oxide nanoparticles are magnetic. Application of magnetic nanoparticles ranges from magnetic storage media to medical industry where they can be used for targeted drug delivery or as contrast agents in MRI. Thus, measuring environmental and biological toxicity of these nanoparticles is crucial. To test toxicity of any such reagents on small organisms, Daphnia and brine shrimps (also known as Artemia) can be used. Artimia are found in salt lakes around the world. They are important live food for aquarium fish. In this lab, you will use brine shrimp to measure toxicity of the nanoparticles. Trypan blue is a stain used to assess cell viability. It is able to diffuse through cell membranes of dead cells but is unable to permeate live cells. This stain can be used to assess cell survival 1 Fall 2017 following exposure to chemicals, nanoparticles and experimental drugs. The ratio of live: dead cells can be used as a preliminary assessment of cytotoxicity and translated to tissue viability.

Methods Part A: Serial Dilution of NP Solution 1) Label 5 test tubes as 0, 1:1, 1:10, 1:100, 1:1000 2) Add 2mL of seawater to tube 0 3) Add 2mL of specific group stock solution to tube 1:1 and add 2mL of seawater to it. 4) Transfer 1mL of stock solution to tube 1:10 and add 9mL of seawater to this tube 5) Transfer 1mL of 1:10 solution to the tube labeled as 1:100 and add 9mL of seawater to 
this tube 6) Transfer 1mL of 1:100 solution to the test tube labeled as 1:1000 and add 9mL of 
seawater to this tube Part B: Preparation of test environments 1) Label 5 wells of 6-well plate as control (0), 1:1, 1:10, 1:100 and 1:1000 2) Label 3 petri dishes as: control (0), 1:1 and 1:1000 
NOTE: Be sure to include your initials on all plates 3) Transfer 2mL of each solution from the corresponding test tube to the respective wells on your six-well plate and each petri dish Part C: Preparing specimens for the Trypan blue cell viability test 1) Transfer 2 brine shrimp into each of the pre-labeled petri dishes containing the solution of NPs and seawater (0, 1:1 & 1:1000) and set a timer for 30 minutes. 2) Transfer a single brine shrimp into the unlabeled petri dish with a drop (~50ul) of seawater and use the dissecting microscope to make observations related to the behavior and morphology of untreated brine shrimp.
Make observations in lab notebook of all samples Part D: Preparation of specimens for the toxicity assay 1) 2) 3) 4) 5) Add 10 brine shrimp into each well in the 6 -well plate Make observations in lab notebook Using a dissecting microscope (if needed), count the number of brine shrimp in each well (alive shrimps).
Record your data in your notebook using the graphic below as a template. Close the plate and store it for next observation.
Observations must be done within 24h-96h
Note: There will be times allocated and a sign up paper for observations Part E: Trypan blue viability test 2 Fall 2017 1) Label 3 glass slides to match the petri dishes prepared in Part C. 2) Transfer 1 brine shrimp from each petri dish onto the corresponding slide. 
Make observations in your lab notebook 3) Add a drop (~60 μl) of PBS on top of each specimen 4) Add 60 μl of Trypan blue to your shrimp and incubate for 1-2 minutes at room 
temperature. 5) Wick away excess liquid 6) Add 100 μl PBS to sample and observe (while alive!), looking for blue cells, using the 
dissecting scope 
Make observations in your lab notebook 7) Label 3 microfuge tubes to align with each sample. 8) Transfer each brine shrimp from the slide into the corresponding microfuge tube 9) Add 50 μl of PBS to the tube and use the mechanical disruption tool to homogenize the 
sample for ~2 minutes until a cell suspension has been generated. 10) Load 30-50 μl of your sample onto a new glass slide and add a cover slip. 11)Observe the sample on high power and use the scale below to qualitatively assess cytotoxicity” 0-no blue 1-some blue 2-fairly blue 3-totally blue.

PREPARE THE JOURNAL ENRTRIES FOR THE FOLLOWING (0NLY FROM TRANSACTON 28 TO 45

During July 2017, the following transactions, prepared by Maddie's Cleaning Service, were completed:
Transaction Date Description
1 July 1 Began her business by contributing a computer valued at $500 and rug cleaner at $6,200 and depositing $2X,XXX (XXXX- represents the last 4 digits of your Bronc's identification number) in a checking account in the name of the corporation in exchange for 6,000 shares, $1 par value shares of capital stock.
2 1 Received $10,000 proceeds from an unsecured, 11%, 5 year, interest only bank loan, due July 1, 2017. The proceeds will be used to pay daily operations of the company. Interest will be paid monthly on the fifth of each month; next payment day is June 5th August 5.
1 Purchased 30 shares of Facebook stock for market rate as of July 1, 2017. MCS paid $12.00 brokerage fee. Use the Short-term Investment (Available For Sale) account.
3 2 Paid $1,500 for 3 floor waxer/buffer machines.
4 2 Purchased a used truck for $18,000 in exchange for a 3 year zero-interest bearing $25,000 note to Jim's Auto Sales.
5 3 Purchased cleaning supplies for $6,500. These supplies will not be resold—use the Cleaning Supplies Inventory account, 2%/15, net 30. The company uses the period inventory method.
6 3 Paid cash for an exclusive 3 yr right to sell cleaning products from Brite N’ Clean Supply company, $8,900.
7 3 Purchased 350 cans of cleaning product, XB4, on account, for resale purposes only, from Brite N’ Clean. Each can costs $8.00. Total shipping costs were $15.00, also included in the amount owed—use the Merchandise Inventory Account.
8 4 Signed a two year lease agreement for storge and office location. Paid 3 months of rent in advance, $3,000, plus security deposit of $1,000, total $4,000
9 5 Paid $4,800 on a one-year insurance policy, effective July 1.
10 7 Hired 5 workers at $15.50 per hour.
11 8 Jim Bob Grocery Store paid Maddie's $5,900 in advance for cleaning services to be performed monthly.
12 12 Billed customers $22,300 for cleaning services.
13 15 Paid gas and oil for the month on the truck, $600.
14 18 Paid amount owed on cleaning supplies purchased on July 3rd.
15 18 Billed customers $16,500 for cleaning services.
16 18 Purchased 40 cans of cleaning product, XB4, $400, for resale purposes only on account. The costs include total shipping costs of $30.00.
17 18 Paid $5,000 to employees, with withholding of $960 for federal income taxes, $150 for state income taxes, $310 for social security, $725 for Medicare tax, and $100 for city income tax. Use Employee Tax Withheld account for all withholding.
18 18 Accrue employer tax (related to July 18th payroll) of $1,352. These taxes include employer portion of FICA and Medicare tax and state and federal unemployment tax. Use Employer Tax Payable account.
19 21 Collected $25,400 from customers billed on July 12.
20 22 Sold 200 cans of cleaning product, XB4, to T. Jones Cleaning on account for $30.00 each. Maddie's’s Cleaning Services, Inc. uses a perpetual inventory method for its merchandise inventory sales and determines costs using LIFO in-first out (LIFO) costing method. Shipping costs were 5%. Costs were charged to T. Jones Cleaning.
21 24 Using Ms. Colbert’s credit card, purchased a used cleaning cart used to carry cleaning supplies to each office, $400.
22 25 Purchased a computer to support office activities, $2,000.
23 30 Bank returned a check for $125 from Tang Juice Company for services paid on July 21st. The bank charges Maddie's’s Cleaning Services $5.00 service fee for the NSF check.
24 30 Paid withholding tax for July 18th payroll, including employer tax.
25 30 Sold one of the floor polisher purchased on July 1 for $850. Record all depreciation related to equipment.
26 31 Declared and paid a $.25 per share cash dividend.
After talking with the client, Ms. Ratcher prepared the following information related to July transactions (use July 31 for the date of transactions):
28 1 Earned but unbilled fees at July 31 were $4,245.
29 2 All equipment is depreciated over 5 years, using the straight line method. Depreciate all equipment as if purchased on July 1.
30 3 An inventory count shows $1,700 of cleaning supplies (not for resale) on hand at July 31.
31 4 Record the amortization of the franchise.
32 5 Accrued but unpaid employee salaries were $5,000 with withholding of $880 for federal income taxes. Calculate the payroll using 6.2% FICA tax, 3% for state income taxes, 1.45% Medicare tax, and 2% for city income tax. Record the payroll, using Employee Tax Withheld account for all withholding taxes.
33 6 Accrued employer payroll tax of $800.00
34 7 Record the rent expiration for the month.
35 8 Record the interest incurred on the note for the month.
36 9 Record amortization of the zero-interest bearing note.
37 10 MCS charged Jim Bob's Grocery $2,800 for services rendered during the month. (See earlier transaction).
39 11 Calculate the change in value of Facebook stock based on market prices as of July 31, 2017.
40 12 One-twelfth of the insurance expired.
41 13 Accrue income taxes for the period. The company expects to pay income taxes at a 18% rate. Note: you must calculate income before you can determine the amount for this adjusting entry.
42 14 Based on an aging of the accounts receivables, Ms. Colbert estimates that 5.5% of outstanding accounts receivables will be uncollectible.
43 Based on the bank reconciliation performed by John, the other new staff member at Daniel and Jacob, LLC, the following adjusting entries should be made:
44 --- A check from Elton Inc. was returned for non sufficient funds (NSF) for $1,000. The check was included in the deposit made on July 21st .
45 --- The deposit made on July 21st for cash receipts was recorded $24,400, but bank correctly counted the deposit to be $25,400.