01:119:117 Study Guide - Final Guide: Pipette, Asepsis, Aqueous Solution
3 Jan 2019
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Serial dilution: stepwise dilution where the stock solution for each dilution is the series diluted from the previous one. Result in series of solutions that are dilute by a certain numerical factor and can be used to make standard curves. % error = [(theoretical mass measured mass)/(theoretical mass)] x 100. Help: is the protein in the buffer? what role does the protein play in relation to the buffer: buffer, protein very dependent on ph, salt conc. And temp: the buffer affects the protein. It must be in the correct ph range, have the right protein"s activity: buffer components concentration, and temperature as stated above, otherwise the protein could be denatured. Important for pcr in master mix: changes of ph from 7. 5 6. 5 of 10 degree 10 fold reduction in the detergents/cofactors = optimize reaction, aqueous solution contains mix of salts, buffer agents, reducing agents, Stock solutions: use to save time and space, usually diluted in water.
