01:447:384 Study Guide - Final Guide: Deoxyribonuclease, Lysosome, Paper Cup
Document Summary
Introduction to pcr the polymerase chain reaction. In lab you were to perform a procedure known as pcr, polymerase chain reaction, to amplify a specific sequence of your own dna in a test tube. You were to look for a particular piece of. Dna called alu that is present in the genes of many but not all people. Analysis of the data generated in this laboratory would enable you to determine whether your genomic dna carries this piece of dna, or not. The objective of pcr is to produce a large amount of dna in a test tube (in vitro), starting from only a trace amount. In other words pcr is used to make millions of copies of target piece of dna. Because significant amounts of a sample of dna are necessary for molecular and genetic analyses, studies of isolated pieces of dna are nearly impossible without pcr amplification.
