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Final

01:447:384 Lecture 17: Fly cross experimentOER14VI18 (1)Exam


Department
Genetic
Course Code
01:447:384
Professor
M.Verzi
Study Guide
Final

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D. melanogaster Mating Experiment: Determining Inheritance of Traits
Dr. Valerie Schawaroch BIO 3015 Principles of Genetics Spring 2018
Initial data collection for this cross experiment.
Phenotype of the P generation listed on the vial says __________________
The date on the vial ____________
The flies you have received are the F1 generation
Take these flies and place them in another vial. You are crossing the F1 generation in this vial.
How many days does it take D. melanogaster at room temperature (25 °C) to create a new
generation (i.e., generation time meaning fertilized egg to winged adult)?____________
Therefore, when should we remove adults to ensure that they do not mate with the emerging
offspring (F2)_by _____________
F1 phenotype of the flies = _______________
Date flies placed in vial ____________
Date flies removed from vial ____________
Date count the emerging offspring, which is the F2 generation _________________
The phenotypes of the flies in the F2 generation are
Work through the handout once all this data is collected to determine the pattern of inheritance
for this trait.
Eden
Chin
(
Female
)
White
/
(
male
)
wild
(
Red
eyed
)
2/07/2020
2
wks
Feb
27
-
March
3rd
Feb
.
2
Oth

Only pages 1-2 are available for preview. Some parts have been intentionally blurred.

D. melanogaster Mating Experiment: Determining Inheritance of Traits
Dr. Valerie Schawaroch BIO 3015 Principles of Genetics Spring 2018
3
X Y
2
4
3
X X
2
Example of wild type female and male D.
melanogaster flies and their respective
chromosomes composition. The autosomes
chromosomes are 2, 3 and 4. Chromosomes 2
and 3 are large metacentric chromosome.
Chromosome 4 is so small it is referred to a
‘dot’ and is a telocentric chromosome. The sex
chromosomes are X which is a are large
acrocentric chromosome and Y which is a
submetacentric chromosome. (Lemeunier and
Aulard, 2000,
Steven J. Baskauf, )
D. melanogaster images taken by Shinhye
Jeon (BIO 3015 Spring 2018) and photoshop
enhanced by V. Schawaroch.
Female
Male
F2 generation
Genotypic
ratio
Phenotypic
ratio
Self crossed
P generation
Red eyed
X
Female fly
White eyed
Male fly
RR
rr
homozygous
homozygous
F1 generation
_______zygous
_______eyed
Both Female & Male flies
F2 generation
Genotypic
ratio
Phenotypic
ratio
Self crossed
P generation
_____ eyed
X
Female fly
_____ eyed
Male fly
homozygous
homozygous
F1 generation
_______zygous
_______eyed
Both Female & Male flies
Now perform the Reciprocal Cross, i.e., the trait of interested is switched between the male and the female from as it
is stated in the initial cross.
This is a thought experiment as well as a wet lab experiment.
About 1910 biological science has rediscovered Gregor Mendel’s experiments and scientists are discussing
Mendel’s ‘factors’ that were hypothesized to be inherited. Sutton and Boveri have been observing cell division and
wondering if the factors might be the chromosomes (or on the chromosomes) which are observed to reduce in
number to produce gametes and then once gametes fuse during fertilization the species number of chromosomes is
restored.
Thomas Hunt Morgan’s lab at Columbia University has decided to use the fruit fly, Drosophila
melanogaster, to learn about the factors affecting the phenotype. Fruit flies have much phenotypic variation and the
trait being studied in this lab is eye color, particularly red eye color and white eye color.
Let’s assume the wild type red eye color and the mutant eye color white are alleles located on an autosome, such as
chromosome 2. In addition, let’s assume red eye color is dominant to white eye color.
Perform the crosses and predict the offspring in the following lineages:
Rr
Rr
l
:2
:L
Red
hetero
3
:/
rr
RR
Rr
Rr
I
:2
:L
Red
white
Red
hetero
3
:
I
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