11:680:390 Study Guide - Final Guide: Lambda Phage, Veterinary Virology, Lysogenic Cycle
27 Apr 2018
School
Department
Course
Professor
GenMicrobio
Dr. R
Exam 3
Open Ended Topics
● Differences between bacteriophages and animal viruses (table + examples)
Bacteriophage
Animal Virus
Attachment
● Cell wall
● Flagella & pillus
● LPS (for gram-negative)
● Cell membrane
Penetration
● injection
● Fusion/Endocytosis
Uncoating
● No uncoating necessary
● Cytoplasm
Synthesis &
Assembly
● Cytoplasm
● Cytoplasm
● Nucleus
Chronic
Infection
● Lysogeny
● Transformation
● Latent infection
● Persistent Infection
Release
● Lysis
● Lytic
● Lysis
● Exocytosis/Budding
Example
● T4
● E. coli virus
● Lambda phage
● Polio
● HIV/HPV
● Antibiotics and cellular targets (similar to matching activity)
○ Antibiotics - naturally produced by microbes to protect its territory; kills/inhibits
microbial growth; target essential molecular processes
ANTIBIOTIC
CELLULAR TARGET/ACTION
Penicillin
Cell wall
Isoniazid
Cell wall - interferes mycolic acid synthesis
Cephalosporins & carbapenems (b-lactams)
Cell wall - interfere with transpeptidation
Document Summary
Differences between bacteriophages and animal viruses (table + examples) Antibiotics and cellular targets (similar to matching activity) Antibiotics - naturally produced by microbes to protect its territory; kills/inhibits microbial growth; target essential molecular processes. Cell wall - prevents new peptidoglycan precursors from reaching site of synthesis. Why rate of pertussis is going up (3 factors) Difference between food poisoning & food infection (+examples) Caused by ingestion of food containing microbial toxins (pathogen does not have to grow in the host) Treatment: just let it run its course. Caused by ingestion of pathogen-contaminated food, followed by growth of pathogen in the host. Coliform examples and how we test (be specific!! Otherwise, you wont get points) - everything on the slide. Potable and recreational water are routinely tested for specific indicator organisms (ex. coliform) signals potential for disease. Coliforms - gram-negative, facultative aerobe, ferments lactose with gas.
