BIO 202 Study Guide - Midterm Guide: Lac Repressor, Lac Operon, Trp Operon

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15 Jan 2017
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G(cid:396)iffith"s e(cid:454)periment: demonstrated the phenomenon of transformation. A(cid:448)e(cid:396)(cid:455)"s e(cid:454)pe(cid:396)i(cid:373)e(cid:374)t: identified the transforming substance as dna. Hershey and chase experiment: determine if the hereditary materiel was dna or protein. Experiments of bacteriophages viruses that infect bacteria. Meselson and stahl semi conservative model. Dna replication (initiation: origin of replication dna replication begins at origins of replication. Dna replication: primase start an rna strand from scratch; and synthesizes rna primer (adds. Rna (cid:374)u(cid:272)leotides(cid:895) at 5" e(cid:374)d of leadi(cid:374)g st(cid:396)a(cid:374)d a(cid:374)d ea(cid:272)h okazaki f(cid:396)ag(cid:373)e(cid:374)t: dna polymerase adds nucleotides o(cid:374)l(cid:455) to the f(cid:396)ee 3" e(cid:374)d of a growing leading strand. Dna helicase unwinds the double helix and breaks the hydrogen bonds b/w strands. Single-stranded binding proteins stabilize the strands and make sure strands stay separated. Topoisomerase binds to the double helix and relieves the strain placed on the double helix as it unravels. Primase binds to site of origin and synthesises rna primer.

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