BCM 475 Study Guide - Midterm Guide: Reactive Intermediate, Competitive Inhibition, Chemical Species
Document Summary
Transition state: chemical species with finite lifetimes (longer than 10-13 s, occur at energy maxima, no measurable lifetime (not finite), some are stable enough to be detected/structurally. Enzyme-product complex (ep: transition between breaking and forming (partially formed/broken) Competitive inhibition | inhibitor resembles substrates binds at active site. Diminishes rate of catalysis (reduce proportion of enzyme molecules bound to substrate) Group-specific reagents | react with specific sides chains of amino acids. Ex: inhibit by covalently modifying a serine residue. Uncompetitive inhibition | inhibitor binds only to enzyme- substrate complex. Binding site for the inhibitor appears when enzyme + Reactive substrate analogs | structurally similar to substrate and covalently bind at active sites. Cannot be opposed by inc. substrate concentration. Noncompetitive inhibition | inhibitor and substrate bind simultaneously to enzyme at different binding sites. Can bind either free enzyme or enzyme-substrate complex. Decrease conc. of functional enzyme (decrease turnover) Suicide inhibitors | modified substrates that provide specific means for modifying active site.
