English ENG 1310 Study Guide - Final Guide: Ketone, Reagent, Ketose
17 Apr 2021
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Title : determination of glucose by the nelson-somogyi"s method. By using the nelson-somogyi"s method, the concentration of glucose and other reducing sugars can be determined. This can be done when the sample extract or test solution is heated together with alkaline tartrate. This will result the reducing of copper from the cupric to cuprous state. Hence, this causes cuprous oxide to be formed. When cuprous oxide is react with arsenomolyybdate reagent, it will formed molybdenum blue which causes the appearance of violet coloured complex. The colour develop is compared with a set of standards in a colorimeter at 500nm. In monosaccharide, all of the carbon atoms except one have a hydroxyl group attached. Monosaccharides can either aldose or ketose based on the position of carbonyl group. Sugar that have aldehyde group is called aldose or aldo sugar while sugar with keto group is called ketose or keto sugar.
