BIO 302 Study Guide - Nuclease, Murine Leukemia Virus, Retina
5 Dec 2014
School
Department
Course
Professor
Document Summary
Worms eat e. coli: small interfering rnas, drosophila at the time, drosophila was a known genome. E. coli and drosophila share many genes enzymes highly conserved. 1: does it produce small dsrna pieces, cg4792 dicer, rnai in drosophila. 2: interferes with gfp expression, rdrp is required for this to work, small bits act as primers introduce transgene into plant. Rdrp will copy regions next to the transgene too. G rxn a rxn w/ some g cleavage t rxn w/ some c cleavage c rxn: electrophoresis. 3: radioautography, read sequence of dna sequencing, sanger method, 1) rxn mixture: Dna polymerase ddntps w/ fluorochromes dntps (datp, dctp, dgtp, dttp) **primer defines sequence specificity*: 2) primer elongation and chain termination, 3) capillary gel electrophoresis separation of dna fragments, 4) laser detection of fluorochromes and computational sequence analysis. ***ssdna bacteriophages*** - package only 1 strand: clone your dna into phage dna now have ssdna sample once obtain from bacteriophage.
