BSCI 283- Midterm Exam Guide - Comprehensive Notes for the exam ( 78 pages long!)
11 Oct 2017
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Newer strand lacks methyl groups: after replication the dna is fully methylated by an enzyme: dna methyl transferase, recombination. Dna uptake, digested into single nts translocasome: 3. Vibrip cholera- grows and divides on surface bloom biofilm on surface of plankton. On sex pilus (similar to type 4 pilus: 2. Pathways: osmotic conditions inhibit ehv2 ompr promoters inhibit promoters, synthetic pathways. Restrictions to bacterial growth: water- limiting factor for growth on skin, iron-limiting factor for intestinal growth restrict trace elements, stationary phase growth does not occur in nature, always in exponential phase but with generation time, critical: nutrient depletion/toxin accumulation. Restrictions to human microbiome: skin-dead skin cells no water, stomach- too high ph, when going from one environment to another can be harmful, normal flora compete with pathogens for nutrients. Benefits of normal microbiota: gnotobiotic- germ free animals. Requirement for vitamins (k and b: some bacteria can cause disease sometimes, but most microbes do not, and many are beneficial to humans.
