BIL 250 Study Guide - Midterm Guide: Restriction Fragment Length Polymorphism, Allele-Specific Oligonucleotide, Variable Number Tandem Repeat
15 Nov 2016
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Random alignment of chrom in meiosis meta i. Single locus/single copy: so only happen in only place. Neutral (in population size/changes over time): no spec function. Microsatellites (str short tandem repeats): good for fingerprinting. Dna misaligns & loops out on new strand = fragment size inc. Dna misaligns & loops out on template = fragment size dec. Rflp (restriction fragment length polymorphisms)- detects polymorphisms using restriction enz + pcr. Synthesize oligonucleotide ms primers & label w/ dye (2) Electrophores ms pcr products on dna seq loaded. 3-4 ms can be multiplexed in each lane with diff dyes. Sequence at least one copy of each allele to verify. Mother & baby = share on allele, father & baby = share other. Must calculate frequency to analyze probability (assay 6-12 loci) Linkage map (restriction site) when dna is cut. Cut w/ different enzyme, each mix loaded sep in agarose gel (-) dna sep by size in electric field (small moves faster)
