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Midterm

BIOL 3113 Study Guide - Midterm Guide: Leucine Zipper, Okazaki Fragments, Dna LigaseExam


Department
BIOL
Course Code
BIOL 3113
Professor
Barbara S
Study Guide
Midterm

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PART I - MULTIPLE CHOICE (80 points - 2 points per question). Answer each question below by placing a
circle around the letter of the response that best answers the question.
1. Which of the following steps in protein synthesis require ATP hydrolysis?
a. joining two amino acids together on the ribosome
b. attaching an amino acid to a tRNA
c. binding of tRNA to the ribosome
d. binding of the ribosome to mRNA
e. base -pairing of codon and anticodon
2. What is the primary limitation of the PCR technique?
a. the ability to isolate DNA of sufficient purity to get a clean sample
b. the difficulty of isolating DNA fragments of sufficient length to get useful copies
c. the need to know at least part of the DNA sequence of the sample
d. the high cost
e. the ability to only analyze repeated sequences
3. A protein with leucine zipper motif is:
a. a DNA-binding protein
b. a chromatin protein
c. a component of the ribosome
d. a hormone.
e. none of the above
4. Initiation of transcription by RNA polymerase II in eucaryotes requires:
a. ligand binding to an inhibitory protein
b. several transcription factors, one of which phosphorylates RNA polymerase
c. the binding of an activator protein to the gene
d. the production of an RNA primer
e. the involvement of a helix-turn-helix protein
5. When two lymphocytes are fused together, which of the following best describes the behavior of the proteins
found in the plasma membranes of the two cells?
a. They are rapidly taken up by coated vesicles
b. The proteins from each cell remain associated with the membrane lipids from the same cell and remain
separated on the surface of the hybrid cell
c. The proteins from each cell diffuse through the bilayer, intermix, and become uniformly distributed over
the surface of the hybrid cell
d. The proteins from each cell form separate patches but do not intermix
e. The proteins from each cell are taken up by endocytosis and degraded in lysosomes
6. What is responsible for proofreading the daughter strand and correcting mistakes during DNA replication?
a. DNA polymerase
b. small nuclear ribonucleoprotein particles
c. DNA primase
d. Okazaki factor
e. DNA ligase

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7. During DNA replication, DNA is synthesized on the lagging strand as:
a. a continuous strand of DNA
b. a discontinuous strands of DNA
c. Okazaki fragments
d. a continuous strands of RNA
e. a discontinuous strands of RNA
8. What seals the nicks in the sugar-phosphate backbone of the DNA that are produced during replication?
a. DNA polymerase
b. DNA primase
c. DNA ligase
d. DNA nickase
e. RNA polymerase
9. A prominent nucleolus indicates that a cell is:
a. getting ready to divide
b. replicating its DNA
c. active in protein synthesis
d. inactive in protein synthesis
e. dying
10. Gene regulatory proteins interact with DNA primarily:
a. by hydrogen bonding with the edges of the nucleotide bases exposed in the major and minor grooves
b. by hydrogen bonding with the sugar - phosphate backbone
c. by disrupting the hydrogen bonding between nucleotide bases from opposite halves of the double helix
d. by hydrogen bonding with nucleotide bases in the center of the helix
e. by binding to RNA polymerase
11. Most transmembrane proteins traverse the lipid bilayer as
a. straight alpha helices, rich in hydrophobic amino acids
b. curved alpha helices
c. mixtures of alpha helices and beta sheets
d. coiled coils
e. globular proteins
12. Which of the following pairs of codons might you expect to be read by the same tRNA as a result of wobble?
a. CUU and UUU
b. GAU and CAU
c. CAC and CAU
d. AAU and AGU
e. CCA and GCU
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13. Which method is used to separate DNA fragments in order to determine the base sequence?
a. ultracentrifugation
b. PAGE
c. agarose gel electrophoresis
d. in situ hybridyzation
e. Western blotting
14. Gene duplication in eucaryotic cells is caused by
a. mutation
b. equal crossing over
c. unequal crossing over
d. viruses
e. DNA polymerase
15. Over time, families of proteins have evolved by the process of:
a. mutation
b. equal crossing over
c. alternative RNA splicing
d. exon shuffling
e. viral infection
16. The DNA base sequence "TATA" is part of the consensus sequence for:
a. the termination sequence for RNA synthesis
b. the promoter sequence for RNA polymerase
c. the initiation site for DNA replication
d. the nucleolar organizer region
e. an enhancer sequence
17 To which part of the ribosome does the mRNA bind?
a. the large subunit
b. the small subunit
c. the A-site
d. the P-site
e. the 50S subunit
18. Negative regulation of gene expression is characterized by:
a. ligand binding to the regulatory proteins.
b. the binding of a repressor protein to the gene
c. the binding of an activator protein to the gene
d. mRNA degradation
e. the binding of a repressor protein to mRNA
19. In situ hybridization can be used to determine:
a. the sequence of the cloned gene
b. the distribution of proteins in tissues
c. the position of the cloned fragment of DNA on the plasmid
d. the size of a gene
e. the distribution of a given type of mRNA in different tissues
20. The enzyme that synthesizes the mRNA is
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