BIO SCI 99 Study Guide - Midterm Guide: Oswald Avery, Photographic Film

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What two ways to denature dna were discussed in class: the opposite of denaturing, in this context, is annealing or hybridization. Hybridization" implies having two strands of nucleic acid polymer from different sources: know how tm is defined and how we can measure it by plotting 260 nm light absorbance at different temperatures. How is tm affected by ph, salt concentration and degree of complementarity between the two strands of the dna duplex: be familiar with the idea of a probe : a labelled segment (or oligonucleotide) of dna or. Rna (usually single-stranded dna) that is complementary to a sequence that you are studying. This oligonucleotide can be labeled at the 5" or 3" end with a fluorescent chemical group or the oligonucleotide can be made to include radioactive atoms, such as phosphorous 32.

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