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BIO SCI 99 Study Guide - Midterm Guide: Phosphodiester Bond, Enzyme, Dnab Helicase

Biological Sciences
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Lecture 7 DNA Replication Part II
(Greyed-out text is meant only to be helpful and is optional)
For the following proteins, know the number present at the replication fork, their
purpose in the replication process, the substrate that they recognize (if applicable),
the reaction(s) they catalyze (if applicable and including the required cofactor, if
applicable), their order in the overall steps of replication (therefore you must know
the steps of replication and their order) and with which of the other proteins they
can directly interact (if applicable):
1) The helicase DnaB
2) SSB
3) The topoisomerase Gyrase
4) Primase
5) The tau complex
6) The clamp loader of the tau complex
7) Beta clamps
Also, in broad terms, how are the beta clamps recycled? Why is this
8) Pol III
9) Pol I
10) Ligase
Could replication of the chromosome still happen if the gene of any of these
enzymes was defective?
Know how the direction of activity is defined for polymerases, helicases and
Understand the experiment described in Figure 11-20b that determined the
direction of activity for DnaB, the replicative helicase.
Understand the ligase mechanism. Understand why ligase is required to create the
phosphodiester bond between the last nucleotide added by Pol I and the first
nucleotide of the previous Okasaki fragment.
What does holoenzyme refer to in the context of the polymerase holoenzyme?
To what does the term replisome refer?
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