BIO SCI 99 Study Guide - Midterm Guide: Nucleotide Excision Repair, Mutation Rate, Base Pair
Course CodeBIO SCI 99
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Lecture 11 DNA Repair Part II
1. Mismatch Repair (MMR)
What is the main purpose of Mismatch Repair? What types of errors can it fix?
What are the basic steps of MMR? What type of enzyme carries out each step?
What is the estimated mutation rate in WT E. coli?
What feature does prokaryotic MMR rely on to determine which base of a
mispaired base pair needs to be replaced? How about in eukaryotes?
2. Repair of damaged bases
What are the different consequences of having damaged bases in the
2.1 Direct Repair
What is direct repair? What two examples did we look at? How does each of the
example direct repair enzymes reverse the chemical damage (in general terms)?
2.2 Excision Repair
2.2.1 Base Excision Repair (BER)
What type of nucleotide damage does BER target? What specific example of BER
was used in lecture? What type of damage is targeted by the example BER
What are the general steps of BER and what type of enzyme catalyzes each
2.2.2 Nucleotide Excision Repair (NER)
What type of nucleotide damage does NER target that BER doesn’t? What
specific example of NER was used in lecture?
What are the general steps of NER and what type of enzyme catalyzes each
In general terms, how is NER targeted to actively transcribed genes? What is the
advantage of doing this?
3. Translesion polymerases
In general, do translesion polymerases repair DNA damage? What is the main
function of translesion polymerases? Is Pol Eta capable of error-free bypass? If so,
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