BIO SCI 97 Study Guide - Final Guide: Ti Plasmid, Organism, Natural Selection

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Genomics: deals with dna sequence, organization, function and evolution of genomes. Made possible by recombinant dna: gene cloning and genetic engineering. Metagenomics: genetic data derived from a community of organisms, usually microbial. Whole genome sequencing: starts with cloning and sequencing of short and random dna fragments. Map-based (clone by clone sequencing): uses a physical map to assemble the fragments before sequencing them. Contigs are continuous sequences of dna assembled by smaller dna fragments that overlap. De novo prediction: mutating a gene and predicting the location of the gene via the known mutation locus. Snp variation (single nucleotide polymorphism): a mutation in a single base pair. This provides the largest number of human diversity. Karyotyping: number of chromosomes in a eukaryotic cell. Transcriptome: number of mrnas in a cell at any given time. This changes as the cell reacts to the environment. **rnaseq: can be used to determine the exons in a gene.

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