MCB 3020C Study Guide - Final Guide: Carbol Fuchsin, Methylene Blue, Gram Staining

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23 Jul 2018
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Lab practical study guide: introduction, safety, and lab techniques, media. Types of media: broth, agar slant, agar deep, or petri dish: microscopy, simple stain, and negative stain, illumination, magnification, and resolution. Total magnification = ocular magnification d (resolution) = lambda / (2na: introduction: staining. Cationic dyes: basic, carry a positively charged chromogen; used to stain the entire actual cell; ex: crystal violet and methylene blue. Anionic dyes: acidic; carry negatively charged chromogen; stains the background of the cells; ex; carbol fuchsin, congo red, and nigrosine. Insoluble: water soluble; ex; india ink: introduction: the negative stain. Used with bright field microscopy to determine size and morphology. Do not ever heat fix this: introduction: the simple stain. One dye stains all cells present; ex: methylene blue or crystal violet: heat fix this, introduction: morphology and arrangement of bacteria, the gram stain, introduction: the gram stain.