# CH ENGR C125 Study Guide - Quiz Guide: Theoretical Plate, Capacity FactorExamPremium

1 pages58 viewsWinter 2019

Department

Chemical EngineeringCourse Code

CH ENGR C125Professor

Anditya RahardiantoStudy Guide

QuizThis

**preview**shows half of the first page. to view the full**1 pages of the document.**Chromatography Homework Problems

1. Based on the graphs below, answer the following:

a) Give an explanation why the peaks in column A are broader than in column B

b) Which column has a greater selectivity factor for compound X and Y?

c) Which column has a greater resolution for X and Y?

2. The following data applies to a column for liquid chromatography.

Length of column is 35.7 cm, the volume of the mobile and stationary phase is 1.6 ml and 0.134

ml, respectively. The column was run at a flow rate of 0.5 ml/min

A mixture of 4 components (A, B, C, D) with the following chromatography data:

Retention time (min Width of Peak Base (min)

Non-retained 2.1 -

A 5.4 0.51

B 10.3 1.03

C 12.1 1.25

D 20.6 1.62

a) If we are interested in component C, what is the number of theoretical plates (N) and

equivalent height of a theoretical plate (H) for the column?

b) Component B and C are the closest component that may affect the separation. Determine

the extent to which each molecule interacts with the stationary phase, the capacity factor

(K’) of B and C.

c) Determine the resolution (R) and the relative retention () between components B and C.

Was good separation achieved?

3. Text book problem No. 7.14

4. Text book problem No. 7.18

Column A

ColumnB

Compound Y

Compound X

Compound X

Compound Y

Unretained

Peak

Unretained

Peak

Response

Time (min)

Response

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