CH ENGR C125 Study Guide - Quiz Guide: Theoretical Plate, Capacity FactorExamPremium
1 pages58 viewsWinter 2019
Course CodeCH ENGR C125
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Chromatography Homework Problems
1. Based on the graphs below, answer the following:
a) Give an explanation why the peaks in column A are broader than in column B
b) Which column has a greater selectivity factor for compound X and Y?
c) Which column has a greater resolution for X and Y?
2. The following data applies to a column for liquid chromatography.
Length of column is 35.7 cm, the volume of the mobile and stationary phase is 1.6 ml and 0.134
ml, respectively. The column was run at a flow rate of 0.5 ml/min
A mixture of 4 components (A, B, C, D) with the following chromatography data:
Retention time (min Width of Peak Base (min)
Non-retained 2.1 -
A 5.4 0.51
B 10.3 1.03
C 12.1 1.25
D 20.6 1.62
a) If we are interested in component C, what is the number of theoretical plates (N) and
equivalent height of a theoretical plate (H) for the column?
b) Component B and C are the closest component that may affect the separation. Determine
the extent to which each molecule interacts with the stationary phase, the capacity factor
(K’) of B and C.
c) Determine the resolution (R) and the relative retention () between components B and C.
Was good separation achieved?
3. Text book problem No. 7.14
4. Text book problem No. 7.18
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