CHEM 153A Study Guide - Final Guide: Citrate Synthase, Isocitrate Dehydrogenase, Citric Acid Cycle
16 Jun 2016
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Citrate synthase is the first step in the tca cycle. Its activity thus determines the rate of the tca cycle. _ directly controlled by the presence of oxaloacetate and acetyl-coa: need for atp (catabolic pathway) Some more sensitive and some less sensitive: need for cac intermediates (product inhibition) (main regulatory mechanism!!) _ citrate and succinyl-coa inhibitors (in the middle of tca cycle, signals how many carbons are actually oxidized in the middle of tca cycle) G o" = -20. 9 kj/mol: energy charge (atp/adp, competitive inhibition. Reduction potential (nadh/nad+) (it can be the intermediate in another anabolic. Accumulation of isocitrate and citrate (as aconitase is operating near equilibrium the build up of isocitrate will cause it to catalyze the reverse reaction. => generate a build up of citrate. => citrate then exported from the mitochondria where tca cycle occurs. Ca2+ activator (trigger muscle contraction in muscle cell. Require high energy that drives tca cycle to produce more e carriers: fad, nadh2,
