MCD BIO 165A- Final Exam Guide - Comprehensive Notes for the exam ( 125 pages long!)

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Study guide: a) what was the question addressed by the experiment shown in the top gel in figure 2b? (1point) In figure 2b we are looking at the western blot from co-immunoprecipitation assays in order to answer the question: do heterologous pdz-pdz interactions restore recruitment of the mutant. Ste5* and the missing kinase: what are the differences between ste5*-ha, ste5*-syn-ha, and ste5-ha? (1 point) The ste5*-ha is the mutated ste5 scaffold (with the ste11 mutation) attached to an epitope ha tag, which is an easily identifiable protein sequence. Ste5*-syn-ha is the mutated ste11 in the ste5 scaffold with an ha tag and also a syn tag. nnos and syn will bind to each other and nothing else in yeast. By fusing the syn tag to the ste5 scaffold and the nnos to ste11 they try to introduce artificial interactions between the scaffold and the mapks.

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