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MCD BIO 90 Study Guide - Quiz Guide: Epigenetics, Blastocyst, Zygote


Department
Molecular, Cell, and Developmental Biology
Course Code
MCD BIO 90
Professor
Mitra Hooshmand
Study Guide
Quiz

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Thursday, October 31, 2019
MCDB 90
Discussion 5!
-Basics!
What is a stem cell?!
Transdierentiation: conversion of one dierentiated cell into another type of
cell. Jellyfish!
Examples of stem cells? toti: zygote; pluri: ESCs; multi: neural stem cells!
-The cell!
Zygote and blastocyst!
Comparing the gene expression profiles of ESCs and iPSCs: mRNA and cDNA
microarray!
RNA ISH: mRNA doesn’t determine transcription, the gene could be post-
transcriptionally regulated. In this case we would test with antibody staining!
Spatial temporal factors, cell-cell interaction, forces!
-Reprogramming and epigenetics!
Reprogramming attempts to remodel the epigenetic pattern of a certain
dierentiated cell. After reprogramming, the cell retains part of its memory in
the epigenetics!
Reprogramming?!
-SCNT: indirect!
-Reprogramming by defined/transcription factors: either!
-Reprogramming by small molecules: either!
-Treatment with pluripotent cell extract: indirect!
-Transplantation!
Nature, nurture, niche!
Xeno-free: No animal product, creating receptors on the human cells, animal
parasite. We cannot use those product on human transplantation!
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