MCD BIO 90 Study Guide - Quiz Guide: Epigenetics, Blastocyst, Zygote
DepartmentMolecular, Cell, and Developmental Biology
Course CodeMCD BIO 90
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Thursday, October 31, 2019
•What is a stem cell?!
•Transdiﬀerentiation: conversion of one diﬀerentiated cell into another type of
•Examples of stem cells? toti: zygote; pluri: ESCs; multi: neural stem cells!
•Zygote and blastocyst!
•Comparing the gene expression proﬁles of ESCs and iPSCs: mRNA and cDNA
•RNA ISH: mRNA doesn’t determine transcription, the gene could be post-
transcriptionally regulated. In this case we would test with antibody staining!
•Spatial temporal factors, cell-cell interaction, forces!
-Reprogramming and epigenetics!
•Reprogramming attempts to remodel the epigenetic pattern of a certain
diﬀerentiated cell. After reprogramming, the cell retains part of its memory in
-Reprogramming by deﬁned/transcription factors: either!
-Reprogramming by small molecules: either!
-Treatment with pluripotent cell extract: indirect!
•Nature, nurture, niche!
•Xeno-free: No animal product, creating receptors on the human cells, animal
parasite. We cannot use those product on human transplantation!
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