BIMM 100- Final Exam Guide - Comprehensive Notes for the exam ( 53 pages long!)

• School: UCSD
• Department: Biology/Molec Biol, Microbiol
• Course: BIMM 100
• Professor: Amy Pasquinelli

Document Summary

If you make a cut through endo(cid:374)u(cid:272)lease, it is a (cid:858)(cid:374)i(cid:272)k(cid:859). If you make a cut, then remove nucleotides within the cut, use endonuclease and exonuclease. Double stranded break in dna: permanently missing genetic information, homologous recombination. Molecular cloning: restriction enzyme: recognize specific sequences to cut, many types of restriction enzymes that can cut specific sequences. Invented by bacteria: developed this mechanism to defend against viral dna. Can distinguish between their own dna and viral dna through methylation. Bacteria can (cid:373)ethylate their o(cid:449)(cid:374) dna so they (cid:449)o(cid:374)"t cut their o(cid:449)(cid:374) dna: can cut gene of interest with restriction enzymes to study its function. Sticky ends will allow you to create the plasmid with the dna of interest. If the mutant can make arginine, you know the circular dna contains the gene that you wanted to isolate. If different restriction enzymes cut a sequence, causing identical overhangs, the strands cut by the enzymes can ligate back together.