MCB 252 Study Guide - Midterm Guide: Thymosin, Phalloidin, Wild Type

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Published on 5 Aug 2020
School
University of Illinois
Department
Molecular and Cell Biology
Course
MCB 252
Professor
!Page 1 of 21!
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!
1.!For!a!homogeneous!polymer!the!critical!concentration!is!the!same!for!both!ends,!and!
the!equilibrium!constant!is!the!same!for!both!ends.!!As!a!result,!the!rate!addition!of!
monomers!at!both!ends!must!be!the!same.!
!
A.!TRUE!
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!
2.!Spectrin!plays!a!role!in!red!blood!cells!and!a!role!in!vesicle!transport.!!As!
discussed!in!lecture,!spectrin!likely!played!a!role!in!vesicle!transport!before!it!
became!adapted!to!its!role!in!red!blood!cells.!
!
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B.!FALSE!
!
3.!!As!described!in!lecture,!because!actin!filaments!are!helical,!any!motor!protein!
that!moves!along!those!filaments!must!follow!a!helical!path.!
!
A.!TRUE!
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!
4.!!As!discussed!in!lecture,!the!number!of!different!human!genes!that!encode!
cytoplasmic!intermediate!filaments!compared!to!the!number!of!cell!types!in!
humans!is!such!that!it!is!consistent!with!a!model!in!which!any!given!cytoplasmic!
filament!is!produced!in!only!3H5!different!cell!types.!
!
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B.!FALSE!
!
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!Page 2 of 21!
5.!As!discussed!in!lecture,!each!of!the!three!types!of!cytoskeletal!polymers!are!polar.!
!
A.!TRUE!
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6.!As!described!in!lecture,!if!you!remove!both!centrioles!from!an!interphase!cell,!that!
cell!cannot!form!new!centrioles!because!a!preHexisting!centriole!is!required!to!
serve!as!a!“template”!for!the!generation!of!new!centrioles.!
!
A.!TRUE!
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!
7. As!described!in!lecture,!for!kinesin!proteins!that!act!as!dimers!to!move!vesicles!
processively!along!microtubules,!both!heads!are!“in!sync”!with!respect!to!their!
ATPase!cycles;!that!is,!when!ATP!is!bound!to!one!head,!ATP!is!also!bound!to!the!
other!head,!and!when!ADP!is!bound!to!one!head,!ADP!is!also!bound!to!the!other!
head.
A. TRUE
B. FALSE
8.!In!lecture!we!discussed!the!fact!that!actin!plays!a!role!in!phagocytosis.!!Based!on!
that!discussion,!do!you!expect!that!addition!of!latrunculin!to!cells!would!stop!
phagocytosis?!
!
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B.!No,!latrunculin!would!not!stop!phagocytosis.!
!
9.!As!discussed!in!lecture,!any!given!human!myosin!II!family!member!is!more!closely!
related!to!(similar!to)!any!human!myosin!I!family!member!than!it!is!to!any!yeast!
myosin!II!family!member.!
!
A.!TRUE!
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10.!As!described!in!lecture,!the!role!of!the!centrioles!is!to!“organize”!the!periH
centriolar!material.!
!
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B.!FALSE!
!
!
!
!
!
!
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!Page 3 of 21!
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11.!Which!of!the!following!is!correct!with!regard!to!the!two!statements!below?!
!
i!=!!For!a!typical!actin!filament!in!a!cell,!the!critical!concentration!for!the!plus!end!is!
higher!than!the!critical!concentration!for!the!minus!end.!
ii!=!As!described!in!lecture,!actin!is!a!homogeneous!polymer!since!it!contains!only!
one!type!of!monomer!protein.!
!
A.!Both!i!and!ii!are!TRUE.!
B.!i!is!TRUE,!and!ii!is!FALSE.!
C.!i!is!FALSE,!and!ii!is!TRUE.!
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12.!In!animal!cells,!vesicles!that!move!toward!the!center!of!the!cell!or!toward!the!
edge!of!the!cell!move!along!which!type(s)!of!filament/polymer?!
!
A.!Actin!filaments!
B.!Intermediate!filaments!
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D.!A!or!B!
E.!B!or!C!
!
13.!What!do!ERM!family!members!do?!
!
A.!Bundle!actin!filaments!in!microvilli!
B.!Link!actin!filaments!together!in!stress!fibers!
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D.!All!of!the!above!(A,!B,!and!C)!
E.!Both!A!and!B!
!
14.!What!triggers!the!conformational!change!in!kinesin!in!which!the!neck!binds!to!
the!head?!
!
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B.!!hydrolysis!of!ATP!to!ADP!+!Pi!!
C.!!ejection!of!Pi!
D.!!release!of!ADP!
!15.!Which!step!in!actin!filament!formation!is!regulated!by!ActA?!
!
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B.!Elongation!
C.!Capping!
D.!Treadmilling!
E.!Disassembly!
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Document Summary

Name:_____________________________ __________ network id:__________________ Section:__________________________ ta:_____________________________ This exam consists of 60 questions for a total of 175 points. Choose the best answer from the available choices. Questions 1- 10 are worth 2 points each. Which step in actin filament formation is regulated by acta: nucleation, elongation, capping, treadmilling, disassembly. Page 3 of 21: the ancestors of some of the humans that migrated out of africa interbred with. Some neanderthal genes are still retained in the human descendants of those interbreeding events. Page 5 of 21: the figure below was discussed in lecture. Refer to the diagram below for the next two questions. Both reactions diagrammed below are for reactions involving the polymerization of actin filaments in an experiment discussed in lecture. Page 8 of 21: as described in lecture both actin filaments and actin monomers have plus and minus ends. You add tubulin, an excess of. Gtp, and any other proteins needed for efficient polymerization of microtubules.