MCB 252 Study Guide - Midterm Guide: Myocyte, Fusion Protein, Chromosome

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Published on 5 Aug 2020
School
University of Illinois
Department
Molecular and Cell Biology
Course
MCB 252
Professor
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NAME:_____________________________'''_________'NETWORK'ID:__________________'
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SECTION:__________________________''''''TA:_____________________________'
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MCB'252'
Exam 3
November 8, 2016
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FORM A KEY
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This'exam'consists'of'60'questions'for'a'total'of'175'points.'
Choose'the'BEST'answer'from'the'available'choices.'
'
Questions'1-10'are'worth'2'points'each.'
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1.!In!lecture!we!discussed!the!fact!that!actin!plays!a!role!in!phagocytosis.!!Based!on!that!
discussion,!do!you!expect!that!addition!of!latrunculin!to!cells!would!stop!phagocytosis?!
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A.'Yes,'latrunculin'would'stop'phagocytosis.'
B.!No,!latrunculin!would!not!stop!phagocytosis.!
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2.!As!discussed!in!lecture,!the!contractile!bundles!(sarcomeres)!found!in!muscle!cells!most!
likely!appeared!in!evolution!before!the!contractile!bundles!found!in!stress!fibers.!
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A.!TRUE!
B.'FALSE!
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3.!!Intermediate!filaments!are!considered!equilibrium!polymers,!as!opposed!to!actin!
filaments!which!are!best!described!as!steady!state!filaments.!
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A.'TRUE'
B.!FALSE!'
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4.!!!As!described!in!lecture,!because!actin!filaments!are!helical,!any!motor!protein!that!
moves!along!those!filaments!must!follow!a!helical!path.!
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A.!TRUE!
B.'FALSE!
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5.!!As!described!in!lecture,!a!polyclonal!antibody!contains!many!different!antibodies,!each!
of!which!recognizes!a!distinct!epitope!on!the!surface!of!an!antigen.!
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A.'TRUE'
B.!FALSE!
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6.!!Profilin!binds!to!which!end!of!an!actin!monomer?!
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A.'The'plus'end'
B.!The!minus!end!
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7.!!!Exon!shuffling!occurs!at!the!level!of!DNA!in!the!genome;!whereas,!splicing!occurs!at!the!
level!of!RNA.!
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A.'TRUE'
B.!FALSE!
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8.!!As!described!in!lecture,!Homo!sapiens!migrated!out!of!Africa!before!Neanderthals!
migrated!out!of!Africa.!
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A.!TRUE!
B.'FALSE!
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9.!!!In!lecture,!we!discussed!the!modular!construction!of!some!genes!and!the!evolution!of!
genes!(and!proteins)!by!the!addition!of!domains.!!As!described!in!lecture,!this!process!
occurs!in!prokaryotes!and!eukaryotes.!
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A.!TRUE!
B.'FALSE!
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10.!!As!described!in!lecture,!all!cytoskeletal!polymers!are!multi-stranded.!
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A.'TRUE'
B.!FALSE!
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Questions'11-55'are'worth'3'points'each.'
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11.!!Which!property!of!eukaryotic!gene!regulatory!regions,!as!discussed!in!lecture,!allowed!
for!the!evolution!complex!regulatory!regions?!
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A.!That!core!promoters!act!over!precise!distances!
B.!That!there!are!different!types!of!core!promoters!
C.!That!eukaryotic!genes!have!a!regulatory!promoter!
D.'That'enhancers'can'act'over'long'distances'
E.!Both!A!and!B!are!correct.!
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12.!Which!of!the!following!statements!is/are!TRUE,!and!which!is/are!FALSE?!
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i!=!!The!majority!of!different!colors!of!fluorescent!proteins!used!by!cell!biologist!are!all!
naturally!occurring!proteins!that!were!isolated!from!different!types!of!aquatic!
organisms.!
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ii!=!As!described!in!lecture,!when!purifying!a!protein!of!interest!one!needs!an!assay!that!is!!
specific!for!that!particular!protein.!
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A.!!Both!i!and!ii!are!TRUE.!
B.!!i!is!TRUE,!and!ii!is!FALSE.!
C.''i'is'FALSE,'and'ii'is'TRUE.'
D.!!Both!i!and!ii!are!FALSE.!
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Document Summary

This exam consists of 60 questions for a total of 175 points. Choose the best answer from the available choices. Questions 1-10 are worth 2 points each: in lecture we discussed the fact that actin plays a role in phagocytosis. As described in lecture, this process occurs in prokaryotes and eukaryotes: true, false, as described in lecture, all cytoskeletal polymers are multi-stranded, true, false. The next two questions are linked: congratulations! You finally managed to build a ray gun that allows you to shrink yourself down to the size of a molecule. Like any normal person, your first idea is to stand on top of a formin protein. That formin protein that you are standing on is immobilized on a solid surface. Actin monomers then start to arrive at the formin upon which you are standing. The arp2/3 complex just nucleated a new actin filament, and that daughter filament has polymerized to add several thousand actin subunits.