BIOLCHEM 415 Study Guide - Final Guide: Van Der Waals Force, Guanidinium Chloride, Succinyl-Coa
Get access
Related Documents
Related Questions
Amino Acid | M.W. g/mol | pKa for side chain |
Alanine | 89.1 | |
Arginine | 174.2 | 12.48 |
Asparagine | 132.1 | |
Aspartate | 133.1 | 3.90 |
Cysteine | 121.1 | 8.37 |
Glycine | 75.0 | |
Glutamate | 147.1 | 4.07 |
Glutamine | 146.1 | |
Histidine | 155.1 | 6.04 |
Isoleucine | 131.2 | |
Leucine | 131.2 | |
Lysine | 146.2 | 10.54 |
Methionine | 149.2 | |
Proline | 115.1 | |
Phenylalanine | 165.2 | |
Serine | 105.1 | |
Threonine | 119.1 | |
Tryptophan | 204.2 | |
Tyrosine | 181.2 | 10.46 |
Valine | 117.1 |
Take a look at your pKa table for the amino acids. It turns out that when these side chains are in a protein, they can have very different pKa values. Explain the following observations:
a. In the enzyme medium chain acyl-CoA dehydrogenase (b-oxidation of fatty acids), E376 has a measured pKa ~ 8.0. It is found in a very hydrophobic environment. What could account for it elevated pKa?
b. For the enzyme aconitase (Krebbâs Cycle), a catalytic serine is known to have a pKa ~ 8. What kind of factors could account for this decrease in pKa?