[BIOLOGY 173] - Midterm Exam Guide - Ultimate 18 pages long Study Guide!

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9/16/2016: homework, print enzyme module, questions at the end - quiz, notes (print, pre-lab assignment due thursday, attached to lecture notes for that week, check the lecture notes each week. If enzyme is limiting, doubling enzyme double reaction rate: need to increase both substrate and enzyme, rate limiting reagent (enzyme, if something is in big excess, it won"t really change much. E + s es ep e + p: finding time (s es) Affinity: if enzyme has high affinity, it"s more attracted to substrate. Goal: take gene from one plasmid and move to another: gene: length of dna, codes for bio useful product, subset of chromo, product = protein or rna. Plasmids: extra chromo genetic elements: circular dna double stranded, replicate independent of chromo control amplification, can be replicated more than chromos, have non-essential genes, natural or engineered can spread antibiotic resistance. Ex. charge, polar/non-polar (hydrophobic/philic), shape bind it to something like a substrate, size.

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