BIOLOGY 173 Study Guide - Midterm Guide: Glycolysis, Dietary Fiber, Ethanol Fermentation

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Microbial cultivation: theory behind spread/streak plating and when to use them. Spread plating involves spreading a small volume of sample evenly over the surface of an agar plate. Theory is that each viable cell from the sample will grow and divide to produce one colony on the agar medium. Report given sample count as the number of colony forming units (cfus) To be successful, you must obtain a countable number of colonies (a good range is 25-250. Cfus); must use serial dilutions to get @ least one plate with optimal cfus. Streak plating is an easy way to obtain pure cultures. Selective media only allow certain types of organisms to grown and they inhibit the growth of other organisms. Differential media are used to differentiate closely related organisms or groups or organisms. With presence of certain dyes or chemicals, the organism will produce characteristic changes or growth patterns used for identification: know how to make serial dilutions.

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