BIOL 202 Study Guide - Midterm Guide: Golden Rice, Penetrance, Endosperm
Document Summary
Recombinant dna technology, restriction endonucleases are especially useful if they generate (cid:498)sticky(cid:499) ends. What: single-stranded complementary tails makes an end sticky, they"ll fit together, vectors such as puc18 others of the puc series contain a large number of restriction enzyme sites clustered in one region. Which term describes this advantageous arrangement of restriction sites: multiple cloning site, assume that a plasmid (circular) is 3200 base pairs in length and has restriction sites at the following locations: 400, 700, 1400, 2600. Give the expected sizes of the restriction fragments following complete digestion: 300, 700, 1000, 1200. Digestion of the same (cid:883). (cid:883) kb fragment with xho) produces two fragments of 3(cid:882)(cid:882) bp and 800 bp. Digestion with both enzymes produces three fragments of 100 bp, 300 bp, and. Therefore, the two genotypes should both occur with equal frequency: independent assortment occurs only in cells that are heterozygous for two genes.
