BIOL 205 Final: BIOL205_Exam3_key_final
Document Summary
Neural crest cells lose e-cadherin and become the peripheral nervous system: [4 points] neural tube cells begin expressing n-cadherin. Neural crest cells do not begin to express anything else; these cells migrate as single cells: a) [4 points] compaction occurs at the 8-cell stage. Therefore, you would want to apply the antibody prior to this stage. If application of antibody prevented compaction, then this would suggest that e-cadherin was necessary for compaction. If injection of e-cadherin induces compaction at this early stage, then it is sufficient to induce compaction: [4 points] you could test for e-cadherin expression via immunocytochemistry using an e- cadherin antibody or use in situ hybridization. Since this is a mouse, you could also use an e- cadherin-gfp reporter. If e-cadherin is involved in compaction, the 8-cell embryo should have. Brachyury: present throughout the mesoderm of the late blastula. Bmp4 receptor: present throughout the mesoderm of the late blastula.
