CHEM 451 Study Guide - Final Guide: Cln3, Phase Boundary, Total Internal Reflection Fluorescence Microscope
Document Summary
Rnps = rna protein complexes, membraneless, comparmentalised within cell without membrane (eg. oil and water don"t mix but still stays in shape), forms membrane that aren"t membrane. Rnp (whi3) has 2 binding partners = cln3 and bn1. Model organism = fungi (lots of nucleus in same cytoplasm) First checked if protein forms assemblies, took whi3 and put it in vitro by itself and put a lot of it. > stuff happens (high conc of whi3 -> not soluble, phase separates because too much of something drives equilibrium/ reached saturation conc so you pop out with your own liquid version) Live imaging using tirf micrscopy, together, so they are liquid. Cln3 + whi3 = nuclear division, bn1 + whi3 = form new branch points off cytoplasm like a tree. Checked whi3 at high conc + no salt -> liquid droplets formed.
