BSCI-1510 Study Guide - Midterm Guide: Peptidyl Transferase, Spliceosome, Eif2
4 Apr 2016
School
Department
Course
Professor
Document Summary
Molecular structure of genes: in bacteria and in eukaryotes. Utr untranslated region before the start codon, or after the stop codon. Alternative splicing: one gene coding for more than one protein. Promoters: (-35) 16-18 bp (-10) 4-7 bp (+1) Rna polymerase: a) core enzyme: a, b,b" w for elongation and termination. b) holoenzyme + sigma for initiation. Sigma subunit binds to promoter (closed complex) Binding energy causes conformational changes (open complex) Catalysis of the first few phosphoester bonds (initiation complex) Conformational changes to dna and protein causes sigma to fall off (elongation complex) Rho independent termination: 2 components: hairpin inverted sequence cagctcgc and poly. A residues (pol falls off when adding us) Promoter: bre (-35) tata (-25) ins (+1) dra (+30) Rna polymerase needs tfii, a, b, d, e, f, g , h. Tfiid binds to tata box through tbp. Formation of first few phosphoester bonds (initiation complex) Conformational changes in protein and dna (elongation complex)
