Biology And Biomedical Sciences BIOL 2960 Study Guide - Quiz Guide: Hydrolysis, Mutagen, Proline
14 Aug 2018
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Protein gel electrophoresis (sds page: gel electrophoresis is used to separate molecules based on some physical characteristic, this lab we separated protein samples using gel electrophoresis. Macpymol: colors and the atoms they represent, yellow-carbon, blue-nitrogen, green-sulfur, helices can be kinked due to a proline reside. Photosynthesis (part 1: red-oxygen, orange-iron, photons are absorbed by pigments in chloroplasts, co2 + h2o + light glucose + o2. Enzyme kinetics of beta-galactosidase: beta-galactosidase catalyzes the hydrolysis of the glycosidic bond to beta-galactose, beta-galactoside can act on lactose to produce glucose and galactose. Dna gel electrophoresis: differences between dna and protein electrophoresis. Does not need to be denatured as there is no secondary structure and it already has a negative charge due to the phosphate groups. Etbr (mutagen) and uv light (can get in-between base pairs-interacting) Bme and heat (reduces disulfide bonds that takes apart quaternary and tertiary structure)
